Specifications.

What CellCount reads, what it returns, and what it runs in. Every figure here is the one printed on the validation page, and every setting is the one the product offers.

Images in

File formats

JPEG, PNG, TIFF

HEIC and HEIF photos are converted to JPEG in the browser on Safari and iOS, the only browsers that can decode them; elsewhere, export the photo as JPEG first.

Resolution

The camera’s full resolution

Frames under about 2,000 px on the short side are too small to count reliably. Images are uploaded as captured: nothing is resized on the way in, because resizing moves the count and compression does not.

Chamber

Improved Neubauer hemocytometer, bright-line ruling

One full 1 mm square, with all four corners, in the frame. The rulings in the image are what the scale is measured from.

Stain

Trypan blue

Clear cells are counted live, blue cells dead.

Focus and exposure

Rulings crisp; detail kept in both the bright and the dark areas

Ways in

A camera on the microscope port

A USB microscope camera, counting live in the browser

The recommended setup. Ask us for a plug-and-play camera matched to your scope.

A phone at the eyepiece

A photo from a modern phone held to the eyepiece; no adapter

Files you already have

JPEG, PNG, TIFF files, one at a time or a whole folder

Including the raw frames an automated counter exports. Raw frames, not the instrument’s overlay images.

The sample

Images per sample

Up to 18 images of one biological sample

Results are given per image and pooled for the sample.

The protocol

Dilution

Any factor; presets up to ×20; one part sample to one part trypan blue is the default

Counting pattern

Which squares of the chamber’s 3 by 3 grid are counted

Sensitivity

High, Balanced, Low

High for small cells such as yeast; Balanced for most samples, including mammalian cells; Low when only confident detections should count.

Cell-size gate

A minimum and a maximum equivalent diameter in micrometres, or off

Sharing

Anyone on the team can create a protocol; every count in the lab follows the one it was made under

The same protocol runs on a chamber image and on a counter’s exported frames.

Versioning

Protocols are versioned; a count records the version it followed

What comes back

Per image

Live and dead counts, with every detected cell marked on the image

Concentration

Cells per mL, from the grid measured in the image and the dilution

Viability

The live share of counted cells, in percent

Size

A cell-size histogram pooled over the sample, with the gate drawn on it

Corrections

Add a missed cell, delete a wrong one, flip live to dead, re-run at another sensitivity

Nothing is final until the person who knows the sample accepts the count.

The record

Acceptance

By a named, signed-in user (their email), at a stated time

Contents

The counts, the grid, the dilution, the protocol with its sensitivity and gate, the model version, the acceptor and the time

Integrity

A SHA-256 digest computed over the record’s exact bytes, stored with it and served with it

Anyone holding the record later can recompute the digest and confirm nothing changed since acceptance.

Documents out

Results document

Sample result, size distribution, per-image table, method, software, statistical analysis, and an appendix of the counted frames with their overlays

Names who accepted the sample and when. Opens in the browser and prints to PDF.

Export bundle

A zip with the original images, the annotated images, the canonical frames and the per-image results as CSV, plus a manifest and a README

For the lab’s own archive; the count can be reviewed later without the app.

Speed and accuracy

Speed

Under 30 seconds per twelve-megapixel image

Live-or-dead accuracy

98.7% on 2,682 held-out cells, scored against expert annotation

Agreement with a leading automated counter

Concordance 0.91 on concentration over 16 images; the same live-or-dead call on 95% of 6,765 cells

The method, the data and every number are kept with the study and are available on request.

Runs in

Browser

Current Chrome, Edge and Firefox; Safari 15 or newer; iOS 15 or newer

Needs WebGL2. Nothing to install.

Devices

Laptop or desktop at the bench, tablet, or phone

Terms

How data is handled is set out in the Privacy Notice and the Terms of Service, and institutions can read the Enterprise Terms. The legal entity on every agreement is Lowkey Vision AS.